Poverty in Philippines Essay

Life is a risking adventure. We commit a lot of obstacles and problems that sometimes pull our hopes down. Watching the documentary creates several feelings about the situations and it brings realizations after and while watching it. First of all, the documentary speaks about a family in Eastern Visayas wherein it reflects a serious poverty in the Philippines. The family experienced or still experiencing sufferings about their whole-life situation. The couples had ten sons and daughters all in all but only six of them were there current priorities. It must be easy for rich families to handle those six responsibilities but for them, it was a serious life challenge. Within a day, eating two meals is the best they can. Green vegetables with rice are considered a delightful meal for them. Because mostly, a piece of bread given by a neighbor or a soup considering rice mixed with water and salt was there food for breakfast while for lunch is still basing for the outcome of their unstable work. They are indeed suffering for poverty – or maybe beyond poverty. As an individual watching an uncommon documentary, I felt a lot of course. First, I felt pity for the whole family but mostly for the children. Children must be playing so free with a full tank – stomach with other kids, educating themselves in school, but what other children of their family do was working too, for the sake of the majority’s food. It was a punch on the heart to see those. Second, I feel so degraded yet fortunate. I am ashamed of myself because they really spend sweats and strengths for their daily benefits while most of us almost take spoon feed from our parents. But I felt so fortunate because even sometimes I feel so poor because of usual money-shortage; I realized how others’ seek hard for a one peso coin. Therefore, the documentary conscientisize most of us, but in a moral way I think. It aroused our conscience not to disgust ourselves because of realizations on mistakes but to change for the better. I am thinking that after watching the documentary, most of the viewer’s felt what I felt too, realized what I realized too, and will change as I’ll change. Starting now, I want to try giving importance with money. I want to encourage myself not to be hopeless in times of struggles. I’ll earn, try my best not to spoil and waste foods, and I will always ask for guidance to God for Him to help me conquer every obstacle. Because life is a risking adventure.

Moliere’s Portrayal of Religion and Religiosity Essay

Moliere’s Tartuffe is about a man named Tartuffe who is a beggar and holy man that was recently taken in by a Christian man named, Orgon. Tartuffe has Orgon fooled, but the entire rest of Orgon’s family sees Tartuffe as a hypocritical, self-righteous con artist. Orgon becomes obsessed with Tartuffe and would rather talk and hear about him than his own sick wife. Even though Moliere rewrote this play twice, there are many reasons that the church might still be concerned about the hypocrisy that he used toward Christianity in this play. In 1666 Tartuffe was banned, a play many found offensive because of its assault on church hypocrisy. Many people of the church could still be concerned, due to Moliere’s portrayal of Christianity and how it is almost mocked in this play. Allot of the comedy Moliere portrays is intense focus on Orgon as example of a certain kind of traditional Christian. He isn’t the ‘only’ type of Christian in this play, but the way he is portrayed is almost in a hypocritical manner. Orgon is so eager to believe Tartuffe, mainly due to the fact that Tartuffe is professing to be another type of Christian. Moliere regards the type of Christian that Tartuffe is as insane. The way mankind is portrayed on behalf of Orgon is almost as depraved as a result of Original Sin, so therefore Orgon has to be subjected, for his own good, to somewhat of a dictatorial control by divinely appointed authorities, or the King. Moliere portrays Orgon as a good Christian man, who is taken advantage of and ultimately almost loses everything because he is willing to take Tartuffe in and treat him as if he were family. The reason the church should be concerned here is the fact that Moliere portrays the way Tartuffe is insane and manipulates the good Christian man. He shows Orgon, the good Christian man as blind to all the wrong, and coming out as essentially the â€Å"loser† and the insane Christian and manipulator, Tartuffe, comes out with all of Orgon’s belongings. After reading Moliere’s Tartuffe I now can see how the church would be heavily concerned with how Moliere portrays Christianity as ‘the nice guy finishes last’ with relation to Orgon and Christianity. The church could also see the hypocrisy by the portrayal of Tartuffe as a â€Å"religious con man† or â€Å"religious fraud. †

LAW 531 Week 1 Knowledge Check Essay

Which of the following is true of a corporation? Corporation owners are only taxed once on earnings. A corporation terminates upon the death of an owner. A corporation is a separate legal entity. Corporation shareholders are subject to unlimited personal liability. Correct 2 There are two general partners, each of whom contributes $5,000 in capital to a limited partnership. There are two limited partners, each of whom contributes $20,000. The total amount of capital contributed is $50,000. The limited partnership agreement does not stipulate how profits and losses are to be allocated. Assume that the limited partnership makes $300,000 in profits. Under the Revised Uniform Limited Partnership Act (RULPA), how much would each partner receive? Each general partner would receive $30,000, and each limited partner would receive $120,000. Each general partner would receive $50,000, and each limited partner would receive $100,000. https://newclassroom3.phoenix.edu/Quiz/Quiz.jsp?launchPresentationR†¦RIS:43590667&serverTime=2013-08-20T04:23:56.746+00:00#/view/result Page 1 of 6 8/19/13 9:24 PM Start Over All partners would receive $75,000, regardless of whether he or she is a general or limited partner. Each general partner would receive $120,000, and each limited partner would receive $30,000. Correct 3 Which of the following is true regarding mediation? A mediator does not make a decision or an award. If a settlement agreement is not reached in mediation, then the parties hire a new mediator. Was created by the Federal Mediation Act of 1925. A settlement agreement is never reached with a mediator. Correct 4 George has served Mary with a complaint alleging breach of contract. Mary has never been sued before and as such, she seeks your advice on what to do with the complaint. You advise that she answer George’s complaint but do not provide any affirmative defenses that George can use against her answer George’s complaint by admitting or denying the allegations George has asserted against her write a letter to the judge saying that George is mistaken should not respond to George’s complaint as an answer implies an admission Correct 5 Which of the following is true of arbitration? A judicial referee makes recommendations to the parties. One party usually drops the case. Parties can introduce evidence to support their case. A resolution may or may not be reached. Correct https://newclassroom3.phoenix.edu/Quiz/Quiz.jsp?launchPresentationR†¦RIS:43590667&serverTime=2013-08-20T04:23:56.746+00:00#/view/result Page 2 of 6 8/19/13 9:24 PM 6 Which of the following is one of the major purposes of a settlement conference? To conduct discovery for a case To contest the local court rules To facilitate the settlement of a case To structure a settlement payment schedule Correct 7 What is the effect of having a corporation as the general partner of a limited partnership? Each shareholder of the corporation will be treated as a limited partner of the limited partnership. The liability of the corporate general partner will be limited to the amount of its assets. The limited liability of the corporation will result in the limited partners having greater liability than they would otherwise. Each shareholder of the corporation will be treated as a general partner of the limited partnership. Correct 8 Which of the following is true about the choice of business entity for an entrepreneur? The choice is determined by the Internal Revenue Service based on all the facts and circumstances. The choice takes into account many factors, including finding an option that has all the characteristics desired. The choice is determined solely by whether the primary business is services or goods. The choice is determined solely by the amount of capital invested. Correct 9 Which form of alternative dispute resolution occurs when the parties choose an impartial third party to hear and decide their dispute? https://newclassroom3.phoenix.edu/Quiz/Quiz.jsp?launchPresentationR†¦RIS:43590667&serverTime=2013-08-20T04:23:56.746+00:00#/view/result Page 3 of 6 8/19/13 9:24 PM Minitrial Arbitration Conciliation Mediation Correct 10 Fred and Ginger are general partners in a business. They decide to purchase a building for the partnership. Ginger will put up the money for the building, and Fred will complete the remodeling. While inspecting the building, Fred is informed that the building is packed full of asbestos. He fails to tell Ginger of the presence of the substance. They buy the building and go into business. During the remodeling of the building, people from the neighborhood begin complaining about the dust from the building. Some of them even threaten to sue. Who is liable? Fred is liable because he was put on notice of the presence of the substance. Ginger is not liable because she did not have actual knowledge. Neither Ginger nor Fred are liable personally, nor is the partnership, as they did not put the substance in the building. Both Ginger and Fred are liable, regardless of the circumstances, by virtue of the fact that they are partners. Ginger is liable because she is the one who purchased the building. Fred is not liable, even though he had actual knowledge, because he did not purchase the building. Correct 11 Martha started a flower shop as a sole proprietor. After 1 year, she was forced to close the shop because business was so bad. At that time, the business assets totaled $50,000, but the business liabilities totaled $125,000. Which of the following statements is true? Martha’s business creditors can collect only the $50,000 of business assets. Once Martha terminates the sole proprietorship, she is no longer liable for the $50,000. Martha’s business creditors can collect only the $50,000 now, but if Martha ever goes into business again, they can get the assets of the new business. Martha is personally liable for the additional $75,000 owed to business creditors. Correct https://newclassroom3.phoenix.edu/Quiz/Quiz.jsp?launchPresentationR†¦RIS:43590667&serverTime=2013-08-20T04:23:56.746+00:00#/view/result Page 4 of 6 8/19/13 9:24 PM 12 Which type of business formation is typically reserved for professionals such as accountants, lawyers, and doctors? Limited Liability Company (LLC) Limited Liability Partnership (LLP) Franchise S-Corporation Correct 13 Which of the following is true in the creation of a general partnership? The name selected cannot indicate that it is a corporation. The business name must have the names of all the partners. The business cannot operate under a trade name. The business name cannot be a fictitious name. Correct 14 Which of the following is true of The Federal Arbitration Act? It permits an appeal for all arbitration awards. It provides that arbitration agreements are valid, irrevocable, and enforceable. It applies only to breach of contract disputes. It governs all types of alternative dispute resolution. Correct 15 When parties agree in advance to adhere to an arbitrator’s decision and award, it is known as binding arbitration https://newclassroom3.phoenix.edu/Quiz/Quiz.jsp?launchPresentationR†¦RIS:43590667&serverTime=2013-08-20T04:23:56.746+00:00#/view/result Page 5 of 6 8/19/13 9:24 PM arbitrator discretion appealable mediation Correct 16 The Black Squirrel limited partnership has been in operation for many years, but has recently fallen on hard times. The partners have decided to dissolve, although there are few assets remaining in the partnership. Shortly after the partnership filed its certificate of limited partnership, the partners had the foresight to incorporate into their partnership agreement a provision that, in the event of dissolution, the assets would be distributed in payment of claims first to limited partners, then to general partners, then to creditors. Hilda is a limited partner and feels relieved that she will receive at least a portion of her capital. Henry, one of the general partners, said that this provision is void and unenforceable. Which of the following best describes this situation? The provision placing limited partners ahead of general partners is unenforceable, thus all partners would be on an equal footing and ahead of creditors. The provision placing the partners ahead of creditors is not enforceable, but the priority of limited partners over general partners is enforceable. The distribution of assets in the event of dissolution is one of the few provisions where the Revised Uniform Limited Partnership Act does not allow modification. The distribution, as called for in the agreement, would be enforceable if it had been included in any filings related to the limited partnership. Incorrect https://newclassroom3.phoenix.edu/Quiz/Quiz.jsp?launchPresentationR†¦RIS:43590667&serverTime=2013-08-20T04:23:56.746+00:00#/view/result

Performance Management Problem Solving Research Paper

Performance Management Problem Solving - Research Paper Example This may occur when an organization releases products to the market without carrying out satisfactory tests for quality. This can cause considerable loss to market share of an organization. Performance management system is important in addressing such organizational problems. Benchmarking forms one of the most crucial components of the performance management system of many organizations. The benchmarking of performance index of products and services, processes, and performance gauges is effectively done using the statistical process control (SPC). The paper will present a discussion of performance index benchmarking by considering the statistical process control (SPC), Six-Sigma concept, and total quality management (TQM), in an effort to realize customer satisfaction. A case example of Hills Industries is discussed to emphasize performance-related problem solving tactics. Problem statement The problem to be addressed was indentified through the process of undertaking a study on the major factors that helps business managers in monitoring and managing their businesses. The study indicates that customer satisfaction or dissatisfaction is the major indicator of market perceptions and trends (Draghici & Petcu, 2010). This leads to the identification of customer dissatisfaction, as a problem, which can be addressed through the six-sigma methodology approach of problem solving. Managers can also identify a problem through time-evaluated approach by visiting places where actual production take place, delivery points or customers’ facilities to gain first-hand observation of how production is carried out and use of manufactured products. The organization can conduct consumer surveys on its products. The organization can select its customers on a random basis and administer questionnaires or conduct telephone interviews in order to gauge its satisfaction with its latest products or services. The company must respond to customer complaints. This is because custom ers tend to evaluate the complaint response in the current condition of dissatisfaction with organizations transactions. Even for customers who are satisfied, failure of transaction can occur. This is an indication that there exists overall evaluation of satisfaction with relationship when the customer encounters a problem transaction or a company’s response to complaint (Riesenberger & Sousa, 2010). Addressing customer dissatisfaction problem requires a problem identification technique that gives an analysis of the current product status. This helps in developing a management intervention useful in guiding the organization towards achieving the desired state in the future. The customer dissatisfaction problem can be identified using the benchmarking, statistical process control (SPC) or a time-evaluated management-by-walking around. SPC is more useful in recurrent manufacturing settings although can also be applicable to any service delivery organization. An effective SPC pr ogram helps managers become aware of how operational processes are performing and anomalies that may arise during the production process, which ultimately affect process outputs. SPC helps to identify flaws in performance. Identification of performance outliers in the course of production may inspire the organization to make necessary changes in the process and product in order to improve customer satisfaction. The SPC helps to benchmark a performance index. The statistical approach must take into account the effects of

New Media Essay Example | Topics and Well Written Essays - 1250 words

New Media - Essay Example With all the praises heaped on the ability of the Internet to empower people, the facts tell us a different story. In 1999, an investigation by the United Nations Development Program found that only 2.5% of the world’s population was connected to the Internet and approximately one quarter of the digitally connected in the world resided in one country – the United States. (cited in Buckingham & Willett 2006, p. 252) More recently, sobering global statistics report have been published by the International Telecommunications Union that of the 141 million Internet hosts across the globe, 106.2 are in the United States and a mere 0.274 million in Africa. (p. 252) Furthermore, while the new media, particularly in the area of information technology, provides us enabling benefits, it is important to underscore that technological developments are themselves shaped by socially bound perceptions of the needs, interests, and purposes that technology serves. According to Judy Wajcman, â€Å"technology is always a form of social knowledge, practices and products. It is the result of conflicts and compromises, the outcomes of which depend primarily on the distribution of power and resources between different groups in society. (cited in Goggin & Newell 2003, p. 3) The growing primacy of commercial motivations for the Internet over access is a case in point. The information superhighway, write James Everett Katz and Ronald E. Rice, is little more than a new medium for corporate American to control and profit from. They argued that the principle behind the national information infrastructure was grounded in privatization and commercialization. (p. 24) Here, access to information is no longer a central presumption of human needs essential to citizenship; rather, consumer interests have become the fundamental requirement in order for the evaluation of the performance of social systems. The technologies that are tagged with

Part 3 Policy Choices Essay Example | Topics and Well Written Essays - 2500 words

Part 3 Policy Choices - Essay Example Third, these new environmental conditions favor the further development of genetic variations within the isolated group. Over a short amount of time, which can last thousands of years in evolutionary paleontology terms, the descendants of the isolated group become a new species morphologically different from the original population (Eldredge & Gould, 1972, p. 94-95; Gould 2002, p. 766-768). As a result, organisms rarely show any gradual evolutionary change throughout their phylogeny. Instead, new species appear quite suddenly in the fossil record. Working from the theoretical insights of Eldredge and Gould, Frank Baumgartner and Bryan Jones used PE to explain their observations on public policy. Baumgartner and Jones (1 993) found that for long periods of observations on different policy subsystems, policy change rarely occurred (p. 17- 18). However, on rare occasions certain policy conditions fluctuated such as the venue for a policy debate or the public's image of a specific policy problem. Fluctuations in venue and image often led to a quick policy change that was immediately followed by additional long periods of policy stasis (Baumgartner & Jones, 1993, p. 38). As a result, Baumgartner and Jones concluded that the evolution of any public policy followed a PE pattern rather than a gradual, incremental pattern. While Frank Baumgartner and Bryan Jones owed much of their insights on PE to Eldredge and Gould, they also used previous research from agenda-setting studies, the policy subsystems literature, and social choice theory to help configure the idea of PE to existing research on public policy. By using these three concepts from social science research, Baumgartner and Jones brought a theory from evolutionary paleontology to political science. This made PE a viable model of the policy process. With the incorporation of agenda-setting, the PE model had its basic political science foundation. Political scientists classify agenda-setting as a debate among advocacy groups, agencies, policymakers, the public, the media, and any other interested organizations over the problems that should be on the active policy agenda of policymakers (Baumgartner & Jones, 1993, p. 10; Kingdon, 1995, p. 3). Once on the agenda, governmental debates over a policy problem occur and the probability of policy change increases. Therefore, agenda-setting becomes important in the PE model because it helps to describe the most fundamental part of policy change. Bringing a policy problem to the agenda is a tough process. Successful agenda-setting usually happens when the public directs the right mix of attention to policymakers on a policy problem that already has various solutions advocated by organized interests (Hunt, 2002, p. 75-76). This helps to change the image of the policy problem from the perspe ctive of policymakers. Such a change in image also causes more policymakers to consider solving the problem with new legislation. When more policymakers know more about a specific policy problem, a change in venue for debates over the problem often occurs. By moving the policy discussion from its usual venue, such as a particular Congressional committee, policy change is more likely to occur. With the interaction of changing images and venues, more people become involved in the policy process and it becomes more open and more susceptible to change. This is an important

Sexual harrasment Essay Example | Topics and Well Written Essays - 1000 words

Sexual harrasment - Essay Example I will rely on specific aspects of sexual harassment and discuss certain laws that relate to sexual harassment in investigating the claim before giving a detailed opinion. Notably, the two women had previously complained to the supervisor’s immediate superior informing him of their discomfort and their need for the unwelcome sexual advances. Ideally, 1995 Auto Corp should have engaged in an investigation process to ascertain the magnitude, facts, and effects of the sexual harassment claim with an aim of resolving the issue to the benefit of the employees and the company. The company should have sought the application of all labor and employment laws to all union and nonunion employees. Although the two female employees were working in the nonunion satellite offices, they enjoyed the protection under Equal Employment Opportunity Commission. The company should have investigated the claim on fair grounds and under the applicable labor and employment laws. In determining the investigator, the company should consider the relationship of the claim and the accused. The investigator should be conversant with the complaint and company policy. The investigator should have established the correctness of the adopted complaint procedure. The investigation on the complaints procedure will have determined the name and position of the accused and other victims. The investigation would also describe the date, location, and witnesses of the incident (SHRM, 2014). The investigation would also establish the effect of the reported claim on the complainant as well as the efforts initiated by the complainant to stop the sexual harassment (SHRM, 2014). Ideally, the internal investigations ought to address the privacy of all parties. The investigator should have interviewed the two female employees, the accused, and any witnesses to help the employer in resolving the complaint (State of

Case Study For Health and Addictive Behaviour Psychology Essay

Case Study For Health and Addictive Behaviour Psychology - Essay Example It is estimated that about 2.6million people suffer from this condition in the UK (Diabetes UK, 2010). There are basically 2 types of diabetes mellitus and they are type-1 and type-2. In type-1 DM, the onset is in young age like childhood, adolescence or even early adulthood. It occurs due to absolute deficiency of insulin as a result of destruction of the beta-cells in the pancreas. On the other hand, type-2 DM occurs mainly in adults, especially in older people and is mainly predisposed by several factors including sedentary lifestyle and obesity. Type-2 accounts for more than 85 percent cases of diabetes (Diabetes UK, 2010). Hereditary factors play an important role in this type of diabetes, either due to genetic predisposition or due to similar behavioral patterns in the families like sedentary lifestyle and eating habits. DM-2 occurs due to a combination of decreased secretion of beta cells in the pancreas and increased peripheral resistance to insulin at tissue-receptor level ( Votey, 2005). DM-2 is the most common type of diabetes. It is managed by pharmacotherapy and appropriate diet and exercise. Diet and exercise have a major role to play in the treatment of diabetes-2. Adam was diagnosed with diabetes type-2 9 months ago. He has been advised to control his blood sugar levels with appropriate diet and exercise, rather than initiating antidiabetic medications. Antihypoglycemic therapy is initiated only when it is not possible to control diabetes through diet and exercise (Diabetes UK, 2010). It is very important to treat and control diabetes because of the notorious complications associated with it. Adam is obese and has been advised to reduce his weight. The main defect in diabetes type-2 is the inability of the tissues to respond to insulin. There is also decreased production of insulin by pancreas. Both these amount to increased glucose levels in the blood, known as hyperglycemia. An important causative factor is obesity. This is more so when obesity is more around the waist, known as central obesity (Votey, 2005). Obesity leads to decreased resistance of tissues to insulin. The fatty acid and triglyceride levels are high and these further interfere with insulin signaling. Another important aspect in obesity which influences the development of diabetes is dysregulation of the secretion of adipokine which is a hormone that causes peripheral resistance to insulin and contributes to the development of diabetes. Thus, obesity has a major role to play in the development of the most common form of diabetes, type-2 diabetes. 1.2 Complications of Diabetes Diabetes leads to increased catabolism and decreased anabolism. After reaching the renal threshold level of 180mg per dl, glucosuria occurs. This contributes to polyuria and polydipsia. Decreased levels of glucose in the cells contributes to delay in the healing of the wounds and also development of recurrent infections. it also causes lipolysis for generation of energy. Lipolysis cau ses an increase in the free fatty acid levels whih are taken up by the liver. Metabolism of free fatty acids in the liver yields ketone bodies, hydroxybutyric acid and acetoacetic acid. As the production of ketone bodies increases, metabolic acidosis ensues, resulting in dehydration. Infact, in many cases, diabetic ketoacidosis is the first presentation and it can turn fata due to development of cerebral edema. Increased lipolysis can

Limited Liability Partnerships Essay Example | Topics and Well Written Essays - 1250 words

Limited Liability Partnerships - Essay Example While a limited company is formulated as a legal entity where limited liability exists, this was not so in the case of partnerships, which were not legal entities and thereby made partners legally liable for all the firm's debts. The Limited Liability Partnership has the following features (a) it has a separate legal personality (b) liabilities of individual members are limited to the amount they agree to pay in the event of an insolvency (c) partners are expected to adhere to the duties and obligations currently imposed upon Directors of Companies (d) the LLP is not expected to hold AGMs or observe the rules for shareholder protection set out in the Companies Act and (e) agreements about how the affairs of the LLP will be run are left to the discretion of individual members, but partners are entitled to share in the management of the firm on an equal basis (Davies, 2001). Dr. Michael Twomey, a partnership lawyer, points out the advantages of a partnership. Firstly, it allows the partners the freedom and flexibility to conduct the Company's affairs as they wish and are not subject to the provisions of Company law. Secondly, they are not required to file accounts; hence their accounts are not publicly scrutinized. Thirdly, since a partnership is not a legal entity, hence no partnership tax is levied and the firm does not pay tax, only the partners do(MOE, 2002). But this singular advantage offered by partnerships, of privacy in the manner in which internal operations of the firm are conducted, is eliminated by way of the LLP, because such privacy can no longer be retained when LLPs are required to file audited accounts at the Companies House and to also disclose information about profits and the share of profits accruing to the highest earning partner (Davies, 2001). As a result, the financial affairs of partnerships will now be brought under public scrutiny and partners will be required to divulge details of profits accrued as well as shares of such profits that are being routed to the partners. One of the reasons why the LLP has been advanced is to mitigate the legal liability of partners for the debts of the firm. This need was especially highlighted in the aftermath of scandals such as Enron, where the Arthur Anderson partners who were not theoretically related to the Enron case, could still be held liable for repayment of debts. But in practice, companies in Britain have not demonstrated any eagerness in coming forward to be incorporated as LLPs. As pointed out by Towmey (MOE, 2002), Clifford Chance, the biggest law firm in London, chose to become an LLP based in America rather than an LLP based in Britian, because in effect, the LLP in Britain has lost all the advantages of financial privacy and flexibility of a partnership. While a partnership was earlier not taxed, most of the principles of Company law have now been applied to it under the LLP format, as a result of which it has to function as if it is a Company rather than a partnership. Thus, it may be noted that the LLP has failed to address one of the most pressing reasons why it was introduced, i.e., the need to ensure that partners are not held liable for the firm's debts. This aspect was vital especially in the case of small businesses where incorporating as a Company is not a feasible option because it involves too much expense and

Company Law Essay Example for Free

Company Law Essay It has been a long established principle of Company Law that the corporate personality is a separate legal entity distinct from its members. (Salomon v Salomon Co. (1897) However, there are circumstances in which the courts might find it appropriate to dispense with this principle and ignore the principle of separate corporate personality by ‘lifting the corporate veil’ so to speak. Yet, the courts have not been as prepared to pierce the veil of the corporation as they have been to protect it. Salomon v Salomon Co. gave birth to the separate legal personality of the corporation. In this case, Mr. Salomon, who was conducting business as a leather merchant formed a company which he called Salomon Co. Ltd in 1892. His shares were distributed among his wife and children, each of whom held one share each, for Mr. Salomon. This was necessary at the time because the law requires that the company consist of at least seven shareholders. It is also important to note that Mr. Salomon was the managing director of the company. (1897) Salomon Co. Ltd. purchased the leather business which Mr. Salomon estimated to be worth 39,000 pounds. Mr. Salomon based this valuation on his view that the business was bound to be a success rather than the actual value at the time of purchase. The funds were paid as follows: 1) 10,000 pounds worth of debenture stocks leaving a charge over all of the assets of the company and 2) 20,000 pounds in 1 pound shares and 9,000 pounds in cash. At this juncture, Mr. Salomon paid off all of the creditors of the business. As a result, Mr. Salomon held 20,001 shares in Salomon Co. Ltd. and his wife and kids held the remaining 6 shares. Also, as a result of the debenture, Mr. Salomon was a secured creditor of the company. (Salomon Salomon Co. Ltd. 1897) The leather business floundered and within a year Mr. Salomon ended up selling all of his debentures so as to salvage the business. This did not work out the way Mr. Salomon planned and the company was unable to pay its debts and consequently went into insolvent liquidation. The company’s liquidator alleged that Salomon Co. Ltd. was nothing but a sham serving as an agent for Mr. Salomon. Therefore Mr. Salomon should be held personally liable for the company’s debts. The Court of Appeal agreed with this finding and held that a company’s shareholders were required to be a bona fide organization with the intention of going into business rather than just for the purpose of meeting the statutory provisions for the number of shareholders. (Salomon Salomon Co. Ltd. 1897) The House of Lords reversed the decision of the Court of Appeal holding as follows:- 1) It was not relevant for the purposes of determining the genuineness of a company’s formation that some shareholders were holding shares for the purpose of forming the company pursuant to relevant statutory provisions. In fact, it was perfectly legal for the procedure for registration to be used by a person for the purpose of conducting a one-man business enterprise. 2) Moreover, a company that was formed pursuant to the regulations provided in the Companies Acts is a separate legal person and was not therefore an agent or trustee for the controller. Therefore the company’s debts were its own and were not the debts of its members. The liability of the members would be limited in proportion to the shares that they each held. (Salomon Salomon Co.  Ltd. 1897) Salomon v Salomon Co. Ltd. has stood up well against the test of time. In Macaura v Northern Assurance Co. [1925] AC 619 the House of Lords held that in the same way that the company’s liabilities are the company’s and the shareholders, the assets are also the company’s rather than the shareholders. (Macaura v Northern Assurance Co. [1925]) In Barings Plc (In Liquidation v Coopers Lybrand (No. 4) [2002] 2 BCLC 364 a parent company suffered a loss as a consequence of the loss incurred by one of its subsidiaries. It was held that the subsidiary was the proper party to commence an action in respect of the loss. This rationale followed the rationale in Salomon v Salomon Co. Vis-a-vis the loss was that of the subsidiary and was therefore that company’s liability rather than the parent company’s liability. The subsidiary was a separate legal entity from its parent company. (2002 p 364) This ruling was closely followed in both Gile v Rhind [2003] as well as Shaker v Al-Bedrawi {2003]. In Re Southard Co Ltd Templeton [1979] 3 ALL ER 556 at 565 LJ said that A parent company may spawn a number of subsidiary companies, all controlled directly or indirectly by shareholders of the parent company. If one of the subsidiary companies, to change the metaphor, turns out to be the runt of the litter and declines into insolvency to the dismay of its creditors, the parent company and other subsidiary companies prosper to the joy of the shareholders without any liability for the debts of the insolvent subsidiary. ’(Re Southard Co Ltd Templeton [1979] 3 ALL ER 556 at 565) Lee v Lee’s Air Farming, a New Zealand case, is another good example of the court’s reluctance to pierce the corporate veil. In this case, in 1954 Lee started a company called Lee’s Air Farming Limited. Lee owned all of the shares of the company and was the company’s Governing Director. In addition, Lee worked for the company as its chief pilot. He died in a plane crash while flying the company plane and his wife tried to claim damages via the company’s insurance scheme under the Workers’ Compensation Act. (Lee v Lee’s Air Farming [1961]) The New Zealand Court of Appeal rejected the widow’s claim that Lee was a worker within the meaning of the Workers’ Compensation Act and the case went to the Privy Council. The Privy Council found that Lee’s Air Farming Limited was an entirely different legal entity from Lee and legal relationships between the two were perfectly permissible. Moreover, the Privy Council found that Lee, as Governing Director could indeed give order to himself in his capacity as chief pilot. Therefore a master/servant relationship did exist between the two and Lee was in that respect a ‘worker’ within the meaning of the Act. Indeed, as seen in the cases discussed above the courts aggressively protect the separate legal identity of the corporate citizen. However, there have been legislative intervention whereby specific situations have been defined where it would be appropriate to pierce the corporate veil. For example Sections 213 and 214 of the Insolvency Acts make it possible for the lifting of the corporate veil in cases of fraud and wrongful dealing. (The Insolvency Act 1986 Sections 213 and 214) Section 213 is often referred to as the ‘fraudulent trading’ provision. (Dignam Lowry 2006 Ch. ) This section arises if the court is satisfied that company carried on any of its business ventures with the intention of defrauding the company’s creditors or the creditors of anyone else. Section 213 will also arise if the court finds that the company acted for any other fraudulent reason and persons involved in those fraudulent ventures can be found liable for the company’s debts. In order to satisfy the court of the existence of fraud Section 213 requires proof of ‘actual dishonesty, involving, according to current notions of fair trading among commercial men, real moral blame’. The . Section 214 does not impose as onerous a burden or standard as does Section 213. It is not necessary to prove an intention to defraud. Section 214 applies to the period just before a company begins winding up procedures. Section 214 arises when the court is satisfied that the directors either knew or ought to have known that the company was becoming insolvent and continued to trade anyway. The director can be liable for the company’s debts in these instances. (The Insolvency Act 1986 Section 214) Section 227 of the Companies Act 1985 makes further provision for lifting the veil of the corporation. This section arises in instances where it is necessary to require the production of group members or group accounts to verify whether or not a subsidiary’s financial activity is that of the holding company. (Companies Act 1985 Section 227) The judiciary has also demonstrated a will to lift the corporate veil whenever the ends of justice desire it to be done. The circumstances in which the court will ignore the corporate veil are ill-defined and the impression is that these circumstances are developed on a case by case basis. Professor Gower said that ‘challenges to the doctrines of separate legal personality and limited liability at common law tend to raise more fundamental challenges to these doctrines, because they are formulated on the basis of general reasons for not applying them, such as fraud, the company being a sham or facade, that the company is the agent of the shareholder, that the companies are part of a single economic unit or even that the interests of justice require this result. ’ (Davies 2003 p 184) Adams v Cape Industries Plc [1990] Ch 433 is viewed by Gower and Davies as the leading case on the exceptions to the corporate veil. In the case the Court of Appeal said that it is not satisfied that the ‘court is entitled to lift the corporate veil as against a defendant company which is a member of a corporate group’ merely on the grounds that the company was used to shield a member of that group from future liabilities of the company. As a matter of fact, the Court of Appeal maintained that this was a legal right by adding ‘whether or not this is desirable, the right to use a corporate structure in this manner is inherent in our corporate law. ’(Adams v Cape Industries Plc [1990] Ch 433) The courts tend to be rather inconsistent with its position on the grounds upon which it will displace the laws protecting the corporate veil. While Adams v Cape Industries Plc was very strict in its position in favor of safeguarding the corporate veil, the House of Lords was rather liberal in DHN Food Distributors Ltd v Tower Hamlets London Borough Council [1976] 1 WLR 852. In the latter case Lord Denning speaking of a parent company and its subsidiary holdings said, ‘these subsidiaries are bound hand and foot to the parent company and must do just what the parent company says’. He went on to say ‘this group is virtually the same as a partnership in which all the three companies are partners. They should not be treated separately so as to be defeated on a technical point’. (DHN Food Distributors Ltd v Tower Hamlets London Borough Council [1976] 1 WLR 852) It wasn’t long before the courts departed from the position taken by Lord Denning. Woolfson v Strathclyde R. C [1978] SLT 159 the House of Lords took issue with Denning’s view on the nature of holding companies and the groups under them. The Lords maintained that the corporate veil would not be displaced unless it was shown that the company was a facade. (Woolfson v Strathclyde R. C [1978] SLT 159) In Trustor AB v Smallbone (No. 2) [2001] 1 WLR 1177 the court was adamant that the corporate veil would only be lifted in three circumstances. They were, 1) if the court was satisfied on the evidence that the company was a mere sham or facade, 2) the company itself was involved in some impropriety or 3) where the interest of justice required it. (Trustor AB v Smallbone (No. ) [2001] 1 WLR 1177) Earlier cases identified appropriate circumstances where the court might find that a company was indeed a facade. In Gilford Motor Company Ltd. v Horne [1933] Ch 985 the court found that the company was a facade. In this case an employee bound by a covenant not to solicit the business of his employers, left his employment and set up a company which he used to breach the covenant. The employee argued that while he was bound by the covenant, the company was not. (Gilford Motor Company Ltd. v Horne [1933] Ch 985) In another case the defendant signed an estate contract with the plaintiff for the sale of realty to him. The defendant changed his mind and formed a company, transferring the realty to the company. He claimed that he was no longer the owner of realty and therefore no bound to the terms of the estate contract. The court found that the company was a mere facade for the defendant and he was ordered to sell the realty as per the estate contract. (Jones v Lipman [1962] 1 WLR 832) The Court of Appeal identified three instances in which it would be appropriate for the corporate veil to be lifted. The court said, ‘save in cases which turn on the wording of particular statutes or contracts, the court is not free to disregard the principle of Salomon v A. Salomon Co Ltd [1897] AC 22 merely because it considers that justice so requires. Our law, for better or worse, recognises the creation of subsidiary companies, which though in one sense the creatures of their parent companies, will nevertheless under the general law fall to be treated as separate legal entities with all the rights and liabilities which would normally attach to separate legal entities. (Adams v Cape Industries Plc [1990] Ch 433) Adams has effectively narrowed the circumstances in which the courts will intervene and lift the corporate veil. This is unfortunate since changing times together with the complex development of both the corporate structure and company law, the Salomon v Salomon Co. rule is in reality perhaps out of place today. (Gallagher Zeigler 1990) Although there have been times when the courts have shifted away from this ruling it remains the poster child for the criteria to be met when determining whether or not to life the veil of the corporation. The prevailing attitude is to safeguard against lifting the corporate veil. Question 2b) The doctrine of majority rule has been a long established principle of Company Law within the English Legal System and makes it difficult for minority shareholders to take legal action in respect of majority shareholder improprieties. That said, Rebecca as a minority shareholder is protected to a limited extent by the provisions of Section 459(1) of the Companies Act 1985. The development of the common law doctrine of majority rule was enunciated in Foss v Harbottle. The rationale behind Foss was that any difficulties within the structure of the company ought to be dealt in the general meetings of the company by ratification by the majority shareholders. The prevailing attitude of the courts was one of nonintervention. It would only step in if it was for the purpose of dissolving the business. The facts of Foss v Harbottle reveal that in 1835 a company, Victoria Park Company purchased land in the Manchester primarily for residential purposes. Thomas Harbottle, a director of Victoria Park Company had purchased the property and resold it to Victoria Park Company who eventually developed the property. Richard Foss and Edward Turton, shareholders of Victoria Park Company brought an action against Thomas Harbottle alleging breach of fiduciary duties in that he sold the property to the company at an inflated price. Turton and Foss also claimed that, acting outside of their powers as directors the directors had burrowed funds in the name of the company. The court held that plaintiffs had no locus standi, and that they were required to have obtained the company’s approval to commence legal action. This approval is properly obtained by virtue of a general meeting. In Foss v Harbottle, Wigram VC explained that ‘the corporation should sue in its own name and in its corporate character, or in the name of someone whom the law has appointed to be its representative. ’ It would therefore only be permissible in exceptional cases of serious abuse that minority shareholders could sue the company as a defendant. This explains the relatively strict approach adopted by the courts in deciding representative forms of actions in the guise of minority shareholder oppression. Jenkins LJ in Edwards v Halliwell explained the justification of the majority rule doctrine in Foss v Harbottle when he said ‘the rule in Foss v Harbottle, as I understand it, comes to no more than this. First, the proper plaintiff in an action in respect of a wrong alleged to be done to a company or association of persons is prima facie the company or the association of persons itself. Secondly, where the alleged wrong is a transaction which might be made binding on the company or association and on all its members by a simple majority of the members, no individual member of the company is allowed to maintain an action in respect of that matter for the simple reason that, if a mere majority of the members of the company or association is in favour of what has been done, then cadit quaestio’. This is where Section 459(1) of the Companies Act 1985 is important to Rebecca in respect of what appears to be ‘insider dealing’, mismanagement and perhaps even fraud. Section 459(1) of the Companies Act 1985 provides as follows:- Any member of a company may apply to the Court by petition for an order under this section on the grounds that the affairs of the company are being or have been conducted in a manner which is unfairly prejudicial to some part of the members (including at least himself) or that any actual or proposed act of omission of the company (including an act of omission on its behalf) is or would be so prejudicial. ’ David Partington, notes rather bluntly, that the discretion contained in Section 459 is very broad and perhaps infinite. ‘The breadth of s. 459 means that there must be an infinite range of situations in which it may be employed. Partington goes on to say that the courts have been extremely flexible in their application of the term ‘unfairly prejudicial. ’ The test for ascertaining whether or not conduct is ‘unfairly prejudicial’ is an objective test rather than a subjective one. The defendant’s motives are often times not of paramount importance to the courts. In Re Bovey Hotel Ventures Ltd. it was held that ‘the test . is whether a reasonable bystander observing the consequences of (the defendants) conduct would regard it as having unfairly prejudiced the petitioners interests. The remedies are no longer limited to ‘winding up’ procedures and this of course explains the wider discretion for commencing an action by minority shareholders. Among the remedies available are, rectification, injunctive or ‘buyout relief. ’ By virtue of ‘buyout’ relief, the court makes an order requiring the company to purchase the shares of the petitioning minority shareholders. This is perhaps the best course for Rebecca to follow. She might not wish to remain a part of a company in which she has all but lost faith in. Re Sam Weller Sons Ltd. rovides some useful guidance as to the kind of conduct that might amount to ‘unfairly prejudicial’ within the meaning of the 1985 Act as amended. For example, failing to pay a dividend in the absence of a sound commercial explanation for such a failure amounts to ‘unfairly prejudicial’ conduct’. In Sam Weller’s case the dividend had already been covered 14 times with the company declaring it for the past consecutive 37 years. In interlocutory proceedings, Gibson LJ denied the company’s application to strike out the petitioner’s claim noting that the company had a case to answer.

Business Level Strategies Versus Corporate Level Strategies Business Essay

Business Level Strategies Versus Corporate Level Strategies Business Essay INTRODUCTION Automobile industry is named the industry of industries by Peter Drucker. Since its humble beginning the automobile industry has involved very much with the times. The industry which is dominated by Europe, US and Japan is currently going through extraordinary changes. After a period of steady growth the industry started to decline in 2008, and declined further in 2009. Currently Toyota holds the majority market share of 10.9 per cent; Volkswagen holds 9.2 per cent share while Ford holds 7.2 per cent of the market share. (Datamonitor, 2009) This report explores the business level and corporate level strategies of Honda Motors and also critically evaluated its process of reconciling dichotomies with reference to product related and process related core competencies. The report examines the Mergers and Acquisitions (MA) in the industry, and the impact of debts, potential synergy and new technology has on MA are investigated with current examples. How automobile players have been dealing with the corporate social responsibilities and its impact on the firms are also discussed. The leadership models by the Japanese management model and Western management model are compared and contrasted. APPLICATION OF STRATEGIES Business Level Strategies Versus Corporate Level Strategies Pizam defined business level strategy as the approach taken by an organization to compete in its chosen markets (2005, pp. 54) Micheal Porter (1980) classified strategies into three generic types; cost leadership, differentiation and focus strategy (cited in Pizam, 2005, pp. 54) Each strategy assists the firm in gaining a certain competitive advantage in a particular competitive scope. However Micheal Porter identified unique features for business level strategies for international businesses (Appendix 1); factors of production, demand conditions, related and supporting industries and firm strategy, structure and rivalry (Hitt, Ireland Hoskisson, 2007, pp. 218) A business level strategy of is its research and development. Hondas product development process is respected within the automobile industry for its sheer speed. Reducing lead time, combining productive efficiency with human dignity, mass production reducing the cost and achieving the expense of product variety are some examples of Hondas RD accomplishments. Honda has a wide international production network; with 89 production facilities (Wit Meyer, 2004, pp. 665) Business level strategies determine the number of plants or production facilities and the locations. Corporate level strategies is to identifies which businesses and industries a company should compete in, which value creation activities it should perform in those businesses and how it should enter of leave those businesses or industries to maximize its long-run profitability (Hill Jones, 2009, pp. 285) Corporate strategy in global context focuses on the scope of diversification, both product and geographical. (Hitt, Ireland Hoskisson, 2009, pp. 220) Hitt, Ireland Hoskisson also states that corporate strategy dictates business level strategies in order to standardize the firms products and sharing of resources across countries (2009, pp. 220) While western management assumes that there is bound to be trade-offs between product quality, cost and delivery; high quality meant high cost etc. However the Japanese developed a right-first-time approach which Honda adopts, which is a corporate strategy dictated to enhance the quality, reduces cost and differentiate the products hence a business level strategy. Honda is the first Japanese firm to manufacture automobiles in the North America when it opened its assembly plant in Ohio is also a business level strategy dictated by corporate level strategy. Other corporate strategies include venturing into the vehicle production from producing auxiliary engines for bicycles and venturing into foreign markets too. While they are product-related diversification here is an example of geographical diversification. Shareholding with European company British Leyland (now known as Rover Group) which was one of the least capable automobile manufacturers is a corporate strategy. Honda has production facilities at 33 countries, (Wit Meyer, 2004, pp. 665) it is also a corporate level strategy of geographical diversification (Peng, 2006, pp. 390) The Managing of Dichotomies by Honda Motor Core competencies refer to firms internal attributes that provide a basis for competitive advantage (Peng, 2006, pp. 77) Core competencies provide access to a wide variety of markets, contributes significantly to end products benefit and are not are not easily imitated. (www.quickmba.com) Prahalad and Hamel define a competence as collective learning in an organization especially how to coordinate diverse production skills and integrate multiple streams of technology (1990, pp. 82) Core competence is often acknowledged in the form of tangible and intangible assets. Organizations should pay equivalent amount of attention to its employees competencies once it starts to pursue core competences track (Bergenhenegouwen et al.,1996) Tangible resources and capabilities are financial, physical, technological and organizational (Peng, 2006, pp. 78) For example, the ability to generate internal funds, ability to raise external capital, location of plants, offices, and equipment, access to raw materials and distribution channels, possession of patents, trademarks and copyrights, formal planning, command and control systems, integrated management information systems (Peng, 2006, pp. 78) Hence tangible resources are those which are directly involved in the manufacturing of a product. Intangible resources and capabilities include human, innovation and reputational (Peng, 2006, pp. 78) Examples of intangible resources are knowledge, trust, managerial talents, organizational culture, a supportive atmosphere for new ideas, research and development capabilities, capacities for organizational innovation and change, perceptions of product quality, durability, and reliability among customers, reputation as a good employer and reputation as a socially responsible corporate citizen (Peng, 2006, pp. 78) These factors are mostly involved in the production process. One of the core competencies Honda is famous for is in the advanced internal combustion engines which power the whole range of its products. The case of CVCC (compound vortox controlled combustion) engine designed so in such a way pollutants are not created. While the rest of the industry was battling with ways to clean up the emitted pollutants, Honda designed so that no pollutants were created to clean in the first place. The dichotomy was reconciled in a way there was no trade-off. Refer to Appendix 2 for the framework to integrate markets, platforms and competencies. In the case of VTEC (variable valve timing and lift electronic control) controlled fuel injection electronically, control the ratio of fuel to air according to driving condition, hence what the process required, hence reconciling the dichotomy of fuel economy vs. engine power. Honda is known for its technologically innovative engines, refusal to accept taken-for-granted trade-offs characterizes all aspects of Hondas strategic approach to technological change (Wit Meyer, 2004, pp. 673) Honda also developed an innovative strategy for the planning of production, a strategy which exhibits characteristics of both the traditional dichotomous poles; one pole is large-lot mass production and the other pole is the one piece flow (Wit Meyer, 2004, pp. 675-6) Hondas own innovative strategy overcame this dichotomy and obtained the advantages of large-lot production which is simpler logistics and quality control, fewer chances of error, easier programming of production schedules. (Wit Meyer, 2004, pp. 676) And also the advantages of small-lot production were obtained, those being ability to offer a wider range of products to consumers and greater worker involvement and satisfaction (Wit Meyer, 2004, pp. 676) MERGERS ACQUISITIONS Mergers are commonly characterised as the consolidation of two organizations into a single organization (Schraeder Self, 2003) Acquisitions are the purchase of one organization from another where the buyer or the acquirer maintains control (Borys and Jemison, 1989) Car-making may be one of the worlds most competitive big industries, but rival producers have always been ready to cooperate on expensive new technologies and products when the cost or risk of going it alone was too high (www.ft.com) The search for partners is now heating as automakers pursue to build scale, lower costs, and pool efforts in zones like small cars, vehicle electrification, and new or emerging markets (www.ft.com) Automotive mergers and acquisitions take place to increase market share, attain economies of scale and augment product ranges (www.reportsure.com) Figure 1; driving factors of Mergers Acquisitions for the years 2007, 2008 2009 Source: KPMGs Global Auto Executive Survey, 2010, pp. 20 Too much Debt and Risk of Bankruptcy Companies believe that a rising rate of MA will be driven partly by crisis factors, and partly by the long-term imperative of finding and developing new technology solutions for a changing market (the continued high stress that companies place upon new technology development KPMGs Global Auto Executive Survey (2010, pp. 20) KPMG predicts that indebtedness will be the top of M A. Also states that all cost pressures now seen as less significant (KPMGs Global Auto Executive Survey, 2010, pp. 20) According to the survey (KPMG Global Executive Survey, 2010), the specific global drivers of alliances, mergers and acquisitions will be driven by crisis factors such as too much debt and risk of bankruptcy 89 per cent (Car Magazine, May, 2010) Ford Motors partnered with Mazda since 1970s to raise cash and to focus on its own brand/ business. (www.ft.com) PSA Peugeot Citroen Toyota produces small cars in Czech Republic. (www.ft.com) As the surveys, too much debt and risk of bankruptcys impact on Mergers and Acquisition (MA) is very high and it is one of the driving forces of MA. Volkswagen in December 2009 announced its plans to buy 19.9 per cent of Suzuki which is to take a stake of approximately 3 per cent of Volkswagen. (www.ft.com) General Motors owned an equity stake in Suzuki from 1991 to 2008 Potential for Product Synergies Companies in auto industry merge to gain product synergies. GM has a contract with Renault to produce vans in Luton, England since 2001 to 2013 (www.ft.com) Daimler announced strategic partnership with Renault and Nissan April 2010; three companies are to build small car engines and vans together. BMW and PSA Peugeot Citroen produce petrol engines together. (www.ft.com) PSA Peugeot Citroen corporates with Mitsubishi Motors on cross over vehicles, electric cars and a joint plant opened in April 2010 in Kaluga, Russia. They also announced that they would work together to develop a compact sport utility vehicle (www.ft.com) Access to New Technologies and Emerging Markets Nissan has OEM (original equipment manufacturer) / supply deals with Mitsubishi, Suzuki, Mazda and Chryslers Dodge brand (www.ft.com) Volkswagen currently owns 49.9% of Porsches sports car business, and Porsche owns 51.1% of Volkswagen. Talk of full-fledged merge is taking place (www.ft.com) Porshe corporates with Volkswagen and its luxury marque Audi sports vehicles. (www.ft.com) PSA Peugeot Citroen with Fiat produces light commercial vehicles and vans. (www.ft.com) New products and new technologies have moved higher among their highest ranking concerns, from an already high leading position in last years survey (KPMG Global Auto Executive Survey, 2010) ROLE OF CSR IN THE AUTOMOBILE INDUSTRY A happy convergence between what your shareholders want and what is best for millions of people Kofi Anan (2001) Corporate social responsibility raises consideration of, and response to, issues beyond the narrow economic, technical, and legal requirements of the firm to accomplish social benefits along with the traditional economic gains which the firm seeks (K. Davis, 1973 cited in Peng, 2006, pp. 484) Carroll (1999, p. 286) suggests that social responsibility covers the conduct of a business so that it is economically profitable, law-abiding, ethical and socially supportive. Global automotive makers and suppliers are also seen to be more corporate responsible now. Going green is a common term in the industry now. Each company in the industry is coming up with a better plan each day. GM has promotes a building greener car, electric car experience and also teach green campaign. (www.gm.com) Toyota has numerous CSR activities as development of environmental vehicles, design for recycling, pedestrian safety campaign, vehicle safety, and customer first, support of art to name a few (www.toyota.com) Refer to Appendix 3 for overview of Toyotas CSR activities. Apart from producing many models of hybrid cars, Honda has devised a Honda LCA (Life Cycle Assessment) system. Honda is determined to measure, assess and analyse the full impact of its products on the environment. Honda LCA will monitor its products entire life cycle from the development and production stages to the final disposal at the end of its life. According to Honda, this will help them identify specific issues at each level of corporate activity and set corresponding targets for action. CSR helps to create shareholder value, increase revenue base, helps in strategic branding, operations efficiency, provides better access to capital, enhance human intellectual capital and also lower business risks (www.csrsingapore.org) Today investors are more aware of corporate governance and social responsibility, associating a firm to these features promote the image of the company and hence better access to capital and other such advantages. Therefore, organizational performance increases. Financial advantages sought in the name of corporate social responsibility are creating shareholders value, increased revenue base, better access to capital and lower business risks. Non-financial benefits are enhanced goodwill of the organisation, strategic branding, and operation efficiency and enhanced human and intellectual capital. While the players of automobile industry focus so much on CSR, the companies are being criticized too. Polonsky and Jevons (2009) states that criticisms has been raised in regarding to global automobile manufacturers who endorse fuel-efficient vehicles of various descriptions, while also producing fuel guzzling SUVs targeted at inner-city users. CSR is a very serious matter. CSR is not only associated with responses to crises, but it has also led to many crises, due to negligence of responsibilities as a corporate citizen. Non-compliance can be expensive and can even push corporations into existence threatening controversy- poor labour relations or public hostility. (Keinert, 2008, pp. 103) And threatening government or civil society action can lead to a slump in sales, lost market share and even withdrawal of capital (Keinert, 2008, pp. 103) LEADERSHIP; JAPANESE STYLE VS. WESTERN STYLE Japanese and American Management are 95% the same, yet differ in all important respects Takeo Fujisawa, founder of Honda Motor Company. In the early 1960s to the 1990s, Japanese management claimed a prominent place in the consciousness and management discourse of the west. Western management involves mass, standardized and fordist production while the Japanese prefer lean, flexible and post-fordist production. A mass production system focuses on producing a single product for a mass market, where else lean production focuses on producing a finite set of standard products designed for a segmented markets demand (Swamidass, 2000, pp. 451) Fordism is the transformation of the economy from agricultural to industrial to mass production to mass consumption. (www.willamette.edu) Where western management model is based at this, the Japanese management mode is post- fordist, the period after that. Mass production equals to fordist standardized production and lean production creates flexible and post-fordist creations. In the work process, western models are taylorist, do workers and unskilled workers. The Japanese work process consists of post-taylor, think workers and polyvalent. Taylorist is a means of detailing a division of labor in time-and-motion studies and a wage system based on performance (www.vanderbilt.edu) which western work process adheres to. The workers are do workers as in, they do what is asked, and thinking is not necessary nor is skilled workers. While the Japanese are post-taylorist, their workers are thinkers, they are to think and come up with ideas for the tasks they are entrusted with. Also under Japanese management style, workers are multi skilled. Production, organization and logistics involve large-lot production, just-in-case method of inventory management and push system. The Japanese management model focuses on, small-lot production, just-in-time and pull system. Just-in-time method is a fool proof method where production, purchasing and distribution all happens just-in-time. In western management model is just-in-case method is more popular. (www.leanmanufacturingconcepts.com) Pull models strive to persistently expand the choices offered while at the same time assisting people to find the resources that are most applicable to them unlike push models treat people as passive consumers (even when they are producers/workers) whose needs can be expected and shaped by centralized decision-makers. (www.johnhagel.com) The organization structure is vertical with fragmented duties and individual as responsible. In the Japanese management model, the structure is horizontal, with broad duties for employees and group is responsible for success of failure. Labour relations are job control focus, cross-company unions and hire and fire strategy. In the Japanese model labour relations are employment conditions focused, enterprise unions and job-for-life employed. Western organizations have separated firms and there are distant inner-firm relations, where else in the Japanese management model it has Keiretsu families and there is close inter-firm relations. There is no better or worse model among the two, as it depends on the situation. And there is no single good method, the situation and the issue in question should be closely examined before choosing the most appropriate model. Just like Honda Motors has, it does not practice one style of management model, the model chosen depends on what the best one is for the firm, given the situation. CONCLUSION The performance of the industry is forecast to accelerate, with an anticipated CAGR of 11.5% for the five year period 2009-2014, which is expected to drive the industry to a value of $2,526.6 billion by the end of 2014 (Datamonitor, 2010)

Essay example --

　　Gathered from the article "Women Get the Vote† in the New York Times, â€Å"Back in July 1776, the Declaration of Independence proclaimed that all men are created equal, but it didn't say anything about women.†(Roberts, Smith), this quote reflects the central idea of the women suffrage movement. The goal was for women to be recognized with the same rights as men, which they achieved (Smiltneek). Thanks to the suffrage movement, America has been forever changed and opened new doors for females. Women of society have evolved into a strong and independent union from the resulting actions of the Women’s Suffrage Movement. 　　Early history, dating back before the women’s suffrage movement, is a key factor to the initiation to the fight for women’s rights. As far back as the Declaration of Independence, women who owned property actually had the right to vote in New Jersey but it took thirty years and that was no longer allowed in the beginning of the 1800’s (Roberts, Smith). The factor that sparked the Women’s Suffrage Movement was Elizabeth Cady Stanton and Lucretia Mott being excluded from the World’s Anti-Slavery Convention due to the fact they were females (Smiltneek). This led the women to composing the Declaration of Sentiment and Resolutions. It is a document laying out the concerns pertaining to women. Seneca Falls, New York marks the inception of the movement for women’s suffrage. This historical phase took place in the year of 1848 when Elizabeth Cady Stanton along with Lucretia Mott endorsed their document. In addition to signing this doc ument, they also composed it. Points they addressed in their testimony included the lack of education arranged for women as well as occupational openings, and laws regarding to custody of childre... ...rought positive enhancements in the duration and afterwards. In Wyoming, women were elected as mayors and an all-women’s fire department was dedicated to them. Other significant effect included Susan Wissle becoming the first mayor of Wyoming in the town Dayton of 1911. Nellie Tayloe Ross was also the first female governor in the whole nation (Layser). This paragraph’s focal point was to visualize some mass effect and historical alterations that would not have occurred at that time if the women’s suffrage movement had not taken place. 　　In conclusion, society’s women have progressed from having limited rights to having privileges they deserved all along, and in doing so, they have achieved a lifetime of rewards. The women’s suffrage movement also defined women gaining more independence by taking control of their rights as well as becoming stronger during this battle.

Identifying Drugs of Abuse Using Gas Chromatography :: Forensic Science

Scenario On the 23rd of September 2015 at 22:58, Mr. Rob Slone was arrested on Sanctuary Road, West Bridgford on possession with intent to supply. A large amount of cash was found on the suspect and a bag containing unknown tablets and powders was recovered from the riverbank, in close proximity to the location of the arrest. A balance with off- white powder, an off- white powder in self sealed bag and a tablet in paper wraps were seized from Mr. Slone's property. At the riverside, a  £5 note, a tablet in paper wraps and 2 off- white powders in self sealed bags, were seized. Physical examination Aim The evidence is physically examined to determine the integrity of the seized substances before any destructive techniques are used. Introduction In forensic science physical examination of the evidence is essential as if the packaging is not correct evidence can be lost or accessed. A chain of custody is important Photography is essential, any photographs should be taken before the evidence is moved. Methods Exhibit MF/2 Before any destructive techniques were used on the exhibit, the integrity of the packaging was determined. The tamper evident bag containing the exhibit was examined to check if was correctly labelled and sealed. Upon receipt of the exhibit the continuity label was signed to ensure the integrity of the exhibit. The tamper evident bag was cut and opened. The yellow paper wrap containing the white tablet measured 1.8x 4cm. The yellow paper wrap was opened to remove the tablet. The exhibit MF/2 was photographed with scale. Visual examination indicates that the exhibit is a white tablet with a manufacturer’s logo CO/COD. The imprint suggested that the tablet could be co-codamol. Co-codamol The tablet was weighed in a plastic weighing boat to prevent any contamination. The tablet weighed 0.6001 grams. All the information was noted on a General Examination Record. Exhibit DR/3 Upon receipt of exhibit DR/3 the continuity label was signed. The tamper evident bag was cut and opened. In the bag there was an off white powder in a sealed bag. The bag containing off white powder was weighed. It weighed 0.80823 grams. Results and discussion Physical examination is essential for the integrity of exhibits. The exhibits must be physically examined before any techniques that would destroy are used. The exhibits The aim of the experiment as a forensic analyst is to examine the seized items visually before the presumptive tests. Exhibits Bag Number Samantha Naas DR/3 D10025448 MF/2 D10025224 Catherine Tuckey DR/2 D10025606 MF/3 D10025590 Lee Berry DR/1 D10025639 MF/4 D10025438 Presumptive tests Aim The presumptive tests are carried out to get a preliminary identification of the controlled sample and are used for qualitative analysis of the unknown substance.

Essay on Male Prejudices in Susan Glaspells Trifles :: Trifles Essays

Male Prejudices in Trifles Susan Glaspell's Trifles explores male-female relationships through the murder investigation of the character of Mr. Wright. The play takes place in Wright's country farmhouse as the men of the play, the county attorney, the sheriff, and Mr. Hale, search for evidence as to the identity and, most importantly, the motive of the murderer. However, the clues which would lead them to such are never found by the men. Instead it is their female counterparts who discover the evidence needed, and who are able to do so because of their gender. The male investigators need to find, as Mrs. Peters puts it, "'a motive; something to show anger, or--sudden feeling'" (357). Yet the men never see the uneven sewing on a quilt Minnie Wright was working on before the murder. The quilt is a symbol of Minnie's agitation--her anger. The men, though, laugh at the women's wonderings about the quilt. To them it is of little importance. Likewise, the bird and its cage are easily dismissed. In fact, the men just as easily believe a lie about this bird and cage. When the cage is noticed, its broken door overlooked, the county attorney asks, "'Has the bird flown?'" Mrs. Peters replies that the "'cat got it'" (360). There is actually no such cat, but the men do not know that and never question the existence of it. The bird, however, is vital to the case. Mr. Wright killed the bird, Minnie's bird, which may have provoked her to then kill him. In addition, the strangling of Mr. Wright, a form of murder which perplexes all when a gun was handy, is reminiscent of the strangling of that bird. It is another answer to the men's questions, but an answer they never find. The women, on the other hand, take note of all they see. They notice not only the bird, the cage, and the quilt but other things that the men call "trifles," like Minnie's frozen preserves and her request for her apron and shawl. These women are united, it seems, not only as country wives or as neighbors but on the basic level of womanhood. This is apparent from the start of the play. Mrs. Hale and Mrs. Peters "stand close together near the door," emotionally bonded throughout the play and, here, physically, in a way, too. Mrs.

Creating Brochure Using Photoshop

Most of us think that creating the brochure is the quite simple task and most of us doesn't think too much of allocating the time to It. But It is not so creating the brochure can make the work cumbersome.. Brochure Is the silent but the most powerful weapon in the marketing field. It helps in promoting the products. So using the time wisely for preparing the brochure makes the work quite simple.Firstly we have to have a clear idea on what is to be included into the brochure, you need to get the specifications of the brochure such as dimensions of paper, trimming, folding, or facial cuts and the most of those things will depend on the budget allocated by the client. Now we shall look how to prepare a brochure In a photos. Firstly the photos software Is to be ready on your system. Ђ Next the piece of paper has to be folded into three sides and then that would make you easy to know which side of the paper would be the main frame and middle and the last frame. Open the new document in the photos in which the dimension as per required. Select the appropriate document which is suitable for the printing out. Ђ Next place all the images that are required In the brochure which makes the brochure attractive. Ђ Place all the matter which Is relevant does not place whole matter In the brochure It should be should precise and sharp such that it should attract the viewers. Next open the other new document same as the above required dimensions that is middle section of your brochure. Now take the print of the first side and place the paper again for the back side and that would finish your brochure.

Marijuana and its Legalization

The legality of marijuana has been a hot topic debated upon for years. However, upon close inspection of studies and articles, it is clear that it is in the better interest of the government to lift the ban on marijuana. The first thing I learned from the inquiry into this issue was that prohibition fails to control the use and production of marijuana. On the contrary, the illegal aspect tickles the curiosity of teenagers, resulting in opportunists seizing this chance to sell marijuana to them.The marijuana is distributed in schools by student dealers who learn to make easy money, and these students may be lead into the crime industries. The problem exists in the ubiquitous nature of marijuana and it not being the highest priority of the police. The distribution is not being controlled and neither are sources of the marijuana, which misses the reason of existence for prohibition.In fact, through legalizing marijuana usage, the government can earn a plethora of money through taxing, t he price can be controlled to be of proper value instead of the underground pricing, and the number of illegal dealers would decrease due to marijuana not being as profitable. In a survey conducted by the National Center on Addiction and Substance Abuse at Columbia University, they reported that â€Å"teens could more readily access pot than beer or prescription drugs. What makes alcohol different from marijuana is that alcohol is legal, regulated through age restrictions, and taught in public education about its awareness. For instance, in high school, there are no such things as alcohol dealers or tobacco dealers, which leads to the need of a middle-man who is of age to buy such products. That middle man is often an older student who has to go out of his way to buy alcohol or tobacco which is a great hassle, whereas marijuana is easily found within the student population.People may argue that it is the pure danger of marijuana that led to its prohibition, but contrary to popular belief, it is said that marijuana was made illegal in the US for population control of Mexicans and Black people. Harry J. Anslinger, the director of the Federal Bureau of Narcotics remarked that the reason for the ban of marijuana was â€Å"its effect on the degenerate races. † There is no scientific evidence that such known side effects as schizophrenia, heart attacks or cancer are actually caused by marijuana and therefore the danger of the drug cannot be the reason being illegal.However, due to marijuana smokers being disoriented and having loss of coordination and logical thought, it would be more reasonable to make certain activities or actions illegal when influenced by marijuana instead, similarly to alcohol. Lastly, the research showed that keeping marijuana illegal is more expensive than lobbying it. Apparently the government’s â€Å"War on Drugs† results in millions of dollars in losses when locking people related to marijuana in prison, paying for th eir food, housing, health care, attorney fees, and court costs.All this is paid by taxpayer money which are meant for and could be used for other things concerning the country. Also, as stated in the above, the government is missing out on tax opportunities that could be in turn used for more effective drug education programs. If marijuana were legal the government would have much more money to spend on important problems. Even in our neighbourhoods, hundreds of students are smoking cannabis. Instead of having a large portion of our population having to smoke illegally, it would be more advisable to free the ban and profit from the perks of legalizing marijuana.

Phylogenetic

Molecular Phylogenetics An introduction to computational methods and tools for analyzing evolutionary relationships Karen Dowell Math 500 Fall 2008 Molecular Phylogenetics Karen Dowell 1 Abstract Molecular phylogenetics applies a combination of molecular and statistical techniques to infer evolutionary relationships among organisms or genes.This review paper provides a general introduction to phylogenetics and phylogenetic trees, describes some of the most common computational methods used to infer phylogenetic information from molecular data, and provides an overview of some of the many different online tools available for phylogenetic analysis. In addition, several phylogenetic case studies are summarized to illustrate how researchers in different biological disciplines are applying molecular phylogenetics in their work. Introduction to Molecular PhylogeneticsThe similarity of biological functions and molecular mechanisms in living organisms strongly suggests that species descended from a common ancestor. Molecular phylogenetics uses the structure and function of molecules and how they change over time to infer these evolutionary relationships. This branch of study emerged in the early 20th century but didn’t begin in earnest until the 1960s, with the advent of protein sequencing, PCR, electrophoresis, and other molecular biology techniques.Over the past 30 years, as computers have become more powerful and more generally accessible, and computer algorithms more sophisticated, researchers have been able to tackle the immensely complicated stochastic and probabilistic problems that define evolution at the molecular level more effectively. Within past decade, this field has been further reenergized and redefined as whole genome sequencing for complex organisms has become faster and less expensive. As mounds of genomic data becomes publically available, molecular phylogenetics is continuing to grow and find new applications. 4, 10, 17, 20, 22] The primary objective of molecular phylogenetic studies is to recover the order of evolutionary events and represent them in evolutionary trees that graphically depict relationships among species or genes over time. This is an extremely complex process, further complicated by the fact that there is no one right way to approach all phylogenetic problems. Phylogenetic data sets can consist of hundreds of different species, each of which may have varying mutation rates and patterns that influence evolutionary change.Consequently, there are numerous different evolutionary models and stochastic methods available. The optimal methods for a phylogenetic analysis depend on the nature of the study and data used. [5, 19, 20] Molecular Evolution: Beyond Darwin Evolution is a process by which the traits of a population change from one generation to another. In On the Origin of Species by Means of Natural Selection, Darwin proposed that, given overwhelming evidence from his extensive comparative analysis of living specimens and fossils, all living organisms descended from a common ancestor.The book’s only illustration (see Figure 1) is a tree-like structure that suggests how slow and successive modifications could lead to the extreme variations seen in species today. [11, 27] Molecular Phylogenetics Karen Dowell 2 Figure 1. Evolution Defined Graphically. The sole illustration in Darwin’s Origin of the Species uses a tree-like structure to describe evolution. This drawing shows ancestors at the limbs and branches of the tree, more recent ancestors at its twigs, and contemporary organisms at its buds. [34] Darwin’s theory of evolution is based on three underlying principles: ariation in traits exist among individuals within a population, these variations can be passed from one generation to the next via inheritance, and that some forms of inherited traits provide individuals a higher chance of survival and reproduction than others. [11] Although Darwin developed his theory of evolution without any knowledge of the molecular basis of life, it has since been determined that evolution is actually a molecular process based on genetic information, encoded in DNA, RNA, and proteins. At a molecular level, evolution is driven by the same types of mechanisms Darwin observed at the species level.One molecule undergoes diversification into many variations. One or more of those variants can be selected to be reproduced or amplified throughout a population over many generations. Such variations at the molecular level can be caused by mutations, such as deletions, insertions, inversions, or substitutions at the nucleotide level, which in turn affect protein structure and biological function. [11, 22] What is a Phylogeny? According to modern evolutionary theory, all organisms on earth have descended from a common ancestor, which means that any set of species, extant or extinct, is related.This relationship is called a phylogeny, and is represented by phyloge netic trees, which graphically represent the evolutionary history related to the species of interest (see Figure 2). Phylogenetics infers trees from observations about existing organisms using morphological, physiological, and molecular characteristics. Figure 2. Phylogeny of Mammalia. This phylogenetic tree shows the evolutionary relationships among six orders of Mammalian species (taxa). Taxa listed in grey are extinct. The â€Å"tree of life† represents a phylogeny of all organisms, living and extinct.Other, more specialized species and molecular phylogenies are used to support comparative studies, test biogeographic hypotheses, evaluate mode and timing of speciation, infer amino acid sequence of extinct proteins, track the evolution of diseases, and even provide evidence in criminal cases. [19] Molecular Phylogenetics Karen Dowell 3 Understanding Phylogenetic Trees Before exploring statistical and bioinformatic methods for estimating phylogenetic trees from molecular data , it’s important to have a basic familiarity of the terms and elements common to these types of trees. See Figure 3. ) Figure 3. Basic elements of a phylogenetic tree. Phylogenetic trees are composed of branches, also known as edges, that connect and terminate at nodes. Branches and nodes can be internal or external (terminal). The terminal nodes at the tips of trees represent operational taxonomic units (OTUs). OTUs correspond to the molecular sequences or taxa (species) from which the tree was inferred. Internal nodes represent the last common ancestor (LCA) to all nodes that arise from that point.Trees can be made of a single gene from many taxa (a species tree) or multi-gene families (gene trees). [1, 10] A tree is considered to be â€Å"rooted† if there is a particular node or outgroup (an external point of reference) from which all OTUs in the tree arises. The root is the oldest point in the tree and the common ancestor of all taxa in the analysis. In the absence of a known outgroup, the root can be placed in the middle of the tree or a rootless tree may be generated. Branches of a tree can be grouped together in different ways. (See Figure 4. ) Figure 4.Groups and associations of taxonomical units in trees. A monophyletic group consists of an internal LCA node and all OTUs arising from it. All members within the group are derived from a common ancestor and have inherited a set of unique common traits. A paraphyletic group excludes some of its descendents (for examples all mammals, except the marsupialia Molecular Phylogenetics Karen Dowell 4 taxa). And a polyphyletic group can be a collection of distantly related OTUs that are associated by a similar characteristic or phenotype, but are not directly descended from a common ancestor. 1, 17] Trees and Homology Evolution is shaped by homology, which refers to any similarity due to common ancestry. Similarly, phylogenetic trees are defined by homologous relationships. Paralogs are homologous s equences separated by a gene duplication event. Orthologs are homologous sequences separated by a speciation event (when one species diverges into two). Homologs can be either paralogs or orthologs. [1, 11, 22] Molecular phylogenetic trees are drawn so that branch length corresponds to amount of evolution (the percent difference in molecular sequences) between nodes. 1, 19] Figure 5. Understanding paralogs and orthologs. Paralogs are created by gene duplication events. (See Figure 5. ) Once a gene has been duplicated, all subsequent species in the phylogeny will inherit both copies of the gene, creating orthologs. Interestingly, evolutionary divergence of different species may result in many variations of a protein, all with similar structures and functions, but with very different amino acid sequences. Phylogenetic studies can trace the origin of such proteins to an ancestral protein family or gene. [1, 22] Figure 6. Mirror Phylogenies.Gene A and Gene A1 are paralogs, whereas all i nstances of Gene A are orthologs of each other in different Canid species. One way to ensure that paralogs and orthologs are appropriately referenced in a phylogenetic tree, and guard against misrepresentation due to missing or incomplete taxonomic information is to generate mirror phylogenies (see Figure 6) in which paralogs serve as each other’s outgroup. [1, 4, 19, 22] Estimating Molecular Phylogenetic Trees Molecular phylogenetic trees are generated from character datasets that provides evolutionary content and context.Character data may consist of biomolecular sequence alignments of DNA, RNA, or amino acids, molecular markers, such as single nucleotide polymorphisms (SNPs) or restriction fragment length polymorphisms (RFLPs), morphology data, or information on gene order and content. Evolution is modeled as a process that changes the state of a character, such as the type of nucleotide (AGTC) at a Molecular Phylogenetics Karen Dowell 5 specific location in a DNA sequence ; each character is a function that maps a set of taxa to distinct states. 1, 19] Note that most of the examples in this paper use DNA sequences as character data, but trees can be accurately estimated from many different types of molecular data. Figure 7. Evolution of a DNA Sequence Figure 7 illustrates how a molecular sequence might evolve over time as a result of multiple mutations that results small, but evolutionarily important changes in a nucleotide sequence. At the protein level, these changes may not initially affect protein structure or function, but over time, they may eventually shape a new purpose for a protein within divergent species. 10, 19, 22] OTUs can be used to build an unrooted phylogenetic tree that clearly depicts a path of evolutionary change. Steps in Phylogenetic Analysis Although the nature and scope of phylogenetic studies may vary significantly and require different datasets and computational methods, the basic steps in any phylogenetic analysis remain t he same: assemble and align a dataset, build (estimate) phylogenetic trees from sequences using computational methods and stochastic models, and statistically test and assess the estimated trees. 4, 19, 20] Assemble and Align Datasets The first step is to identify a protein or DNA sequence of interest and assemble a dataset consisting of other related sequences. For example, to explore relationships among different members of the Notch family of proteins, one might select DNA sequences for Notch1 through Notch4, in different species, such as human, dog, rat, and mouse, then perform a multiple sequence alignment to identify homologies. [1, 10, 13, 19, 20] There are a number of free, online tools available to simplify and streamline this process. DNA sequences of interest can be retrieved using NCBI BLAST or similar search tools.When evaluating a set of related sequences retrieved in a BLAST search, pay close attention to the score and E-value. A high score indicates the subject seque nce retrieved with closely related to the sequence used to initiate the query. The smaller the E-value, the higher the probability that the homology reflects a true evolutionary relationship, as opposed to sequence similarity due to chance. As a general rule, sequences with E-values less than 10-5 are homologs of a query sequence. [10] Once sequences are selected and retrieved, multiple sequence alignment is created.This involves arranging a set of sequences in a matrix to identify regions of homology. Typically, gaps (one or more spaces in the alignment) are introduced in one or more sequences to represent insertions or deletions in the molecular code that may have occurred over time. Effective multiple sequence alignment hinged on gap analysis—determining where to insert gaps and how large to make them. There are many websites and software programs, such as ClustalW, MSA, MAFFT, and T-Coffee, designed to perform multiple sequence on a given set of molecular data. ClustalW i s currently the most mature and most widely used. 1, 10. 19] Molecular Phylogenetics Karen Dowell 6 Building Phylogenetic Trees To build phylogenetic trees, statistical methods are applied to determine the tree topology and calculate the branch lengths that best describe the phylogenetic relationships of the aligned sequences in a dataset. Many different methods for building trees exist and no single method performs well for all types of trees and datasets. The most common computational methods applied include distance-matrix methods, and discrete data methods, such as maximum parsimony and maximum likelihood. 4, 17, 20] There are several software packages, such as Paup*, PAML, PHYLIP, that apply most popular methods. [4] Paup* is a commercially available program that implements a wide variety of methods for phylogenetic inference, including maximum likelihood analysis for DNA data using different models. Paup* also includes a set of exact and heuristic methods for searching optimal trees. PAML (Phylogenetic Analysis by Maximum Likelihood) is open-access set of programs for phylogenetic analysis and evolutionary model comparison.PAML includes many advanced models—DNA- and AAbased models as well as codon-based models that can be used to detect positive selection. Many of the programs in PAML can model heterogeneity of evolutionary rates among sequence sites using ? distributions, and evolutionary dynamics of different sequence regions (concatenated gene sequences). PHYLIP is another large suite of open-access programs for phylogenetic inference that estimates trees using numerous methods, including pairwise distance, maximum parsimony, and maximum likelihood.The maximum likelihood programs can handle a few simple stochastic models and have good tree searching capabilities. PHYLIP is generally considered good educational software for novice phylogeneticists. Distance-Matrix Methods Distance matrix methods compute a matrix of pairwise â€Å"distances† between sequences that approximate evolutionary distance. Distance-based methods tend to be in polynomial time and are quite fast in practice. These methods use clustering techniques to compute evolutionary distances, such as the number of nucleotide or amino acid substitutions between sequences, for all pairs of taxa.They then construct phylogenetic trees using algorithms based on functional relationships among distance values. There are several different distance-matrix methods, including the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA), which uses a sequential clustering algorithm; the Transformed Distance Method, which uses an outgroup as a reference, then applies UPGMA; the Neighbor-Relations Method, which applies 4point condition to adjust the distance matrix, then applies UPGMA; and the Neighbor-Joining Method, which arranges OTUs in a star, the finds neighbors sequentially to minimize total length of tree. 4, 17] The following section on the UPGMA method prov ides a more detailed example of how distance-matrix methods work. UPGMA Method UPGMA produces rooted trees for which the edge lengths can be viewed as times measured by a molecular clock with a constant rate. This method uses a sequential clustering algorithm to identify two OTUs that are most similar (meaning they have the shortest evolutionary distance and are most similar in sequence) and treat them as a single new composite OTU. This process is repeated iteratively until only two OTUs remain.The algorithm defines the distance (d) between two clusters Ci and Cj as the average distance between pairs of sequences from each cluster: Molecular Phylogenetics Karen Dowell 7 Where |Ci| and |Cj| are the number of sequences in clusters i and j. This sequential clustering process is visually described in Figure 8. In this example, the two most homologous sequences are 1 and 2. They are clustered into a new composite parent node (6), and the branch lengths (t1 and t2) are defined as 1/2d1,2 . The next step is to search for the closest pair among remaining sequences and node 6.Pair 4 and 5 are identified and clustered into a new parent node (7), and the branch length for t4 and t5 is calculated. [4, 17] Figure 8. Sequential clustering of sequences using the UPGMA method. [17] In this interactive process, parent node 8 is created from pairs 7 and 3, and parent node 9 is created by clustering nodes 6 and 8. [4, 17] Thus, all sequences are clustered into a single evolutionary tree. The total time (t9) can be calculated as: D6,8 = 1/6 (d1,3 + d1,4 + d1,5 + d2,3 + d2,4 +d2,5)Discrete Data Methods Discrete data methods examine each column of a multiple sequence alignment dataset separately and search for the tree that best represents all this information. Although distance-based methods tend to be much faster than discrete data methods, they typically yield little information beyond the basic tree structure. Discrete data analyses, on the other hand, are information rich. The se methods produce a separate tree for each column in the alignment, so it is possible to trace the evolution for specific elements within a given sequence, such as catalytic sites or regulatory regions. 10, 17, 19, 20) Commonly used discrete data methods include maximum parsimony, which searches for the most parsimonious tree that requires the least number of evolutionary changes to explain differences observed, maximum likelihood, which requires a probabilistic model for the process of nucleotide substitution, and Bayesian MCMC, which also requires a stochastic model of evolution, but creates a probability distribution on a set of trees or aspects of evolutionary history. [17, 19, 20] Discrete data methods are generally considered to produce the best estimates of evolutionary history.However, these methods can be computationally expensive, and it can take weeks or months to obtain a reasonable level of accuracy for moderate to large datasets with 100 or more OTUs. [19] Molecular P hylogenetics Maximum Parsimony Karen Dowell 8 Among the most widely used tree-estimation techniques, maximum parsimony applies a set of algorithms to search for the tree that requires the minimum number of evolutionary changes observed among the OTUs in the study. For example, Figure 9 lists four sample sequences from which phylogenetic trees could be inferred using maximum parsimony.Site Seq 1 2 3 4 1 A A A A 2 A G G G 3 G C A A 4 A C T G 5 G G A A 6 T T T T 7 G G C C 8 C C C C 9 A G A G Figure 9. Sample sequences for a maximum parsimony study [17] Maximum parsimony algorithms identify phylogenetically informative sites, meaning the site favors some trees over others. Consider the sequences in Figure 9: Site 1 is not informative, because all sequences at that site (in column 1) are A (Adenine), and no change in state is required to match any one sequence (1-4) to another.Similarly, Site 2 is not informative because all three trees require one change and there is no reason to favor one tree over another. Site 3 is not informative because all three trees require two changes. (See Figure 10). Figure 10. Site 3 trees all require one evolutionary change. [17] Site 4 is not informative because all three trees require three changes. No one tree can be identified as parsimonious. (See Figure 10 Figure 11. Site 4 trees all require three evolutionary changes. [17] Site 5 is informative because one tree requires only one nucleotide change, whereas the other two trees require 2 changes.In Figure 12, the first tree on the left, which requires only one nucleotide change, is identified as the maximum parsimony tree. Figure 12. Site 5 trees vary in the number of evolutionary changes required. [17] Molecular Phylogenetics Maximum Likelihood Karen Dowell 9 The maximum likelihood method requires a probabalistic model of evolution for estimating nucleotide substitution. This method evaluates competing hypotheses (trees and parameters) by selecting those with the highest likeliho od, meaning those that render the observed data most plausible. The ikelihood of a hypothesis is defined as the probability of the data given that hypothesis. In phylogeny reconstruction, the hypotheses are the evolutionary tree (its topology and branch lengths) and any other parameters of the evolutionary model. [17, 20] The likelihood calculations required for evolutionary trees are far from straightforward and usually require complex computations that must allow for all possible unobserved sequences at the LCA nodes of hypothesized trees. This method specifies the transition probability from one nucleotide state to another in a time interval in each branch.For example, for a one-parameter model with rate of substitution ? per site per unit time, the probability that the nucleotide at time t is i is: The probability that the nucleotide at time t is j is: To set up a likelihood function, given x as the ancestral node and y and z as internal nodes, the probability of observing nucle otides i, j, k, l at the tips of the tree is computed as: Pxl(t1+t2+t3)Pxy(t1)Pyk(t2+t3)Pyz(t2)Pzi(t3)Pzj(t3) For the ancestral node (root) x, the probability of having nucleotide l in sequence 4 is calculated as: Pxl(t1+t2+t3)Because x, y, and z can be any one of four nucleotides (ACGT), it is necessary to sum over all possibilities to obtain the probability of observing the configuration of nucleotides i, j, k, l, in sequences 1, 2, 3, 4, for a given hypothetical tree (see Figure 13. ). This likelihood probability is calculated as: h(I,j,k,l)= [? gxPxl(t1+t2+t3)] [? Pxy(t1)Pyk(t2+t3)] [? Pyz(t2)Pzi(t3) Pzj(t3)] The appropriate likelihood function depends on the hypothetical tree and the evolutionary model used. (See Figure 13. ) [17] Figure 13. Different types of model trees for the derivation of the maximum likelihood function. 17] Molecular Phylogenetics Stochastic Models of Evolution Karen Dowell 10 Evolutionary changes in molecular sequences result from mutations, some of whic h occur by chance, others by natural selection. Rates of change can also differ among OTUs, depending on several factors ranging from GC content to genome size. To accurately estimate phylogenetic trees, assumptions must be made about the substitution process and those assumptions must be stated in the form of a stochastic evolutionary model. These probabilistic models are used to rank trees according to likelihood: P(data|tree).From a Bayesian perspective, they rank trees according to a posterior probability: P(tree|data). [17, 20] The objective of probabilistic models is to find likelihood or posterior probability of a particular taxonomic feature, then define and compute: P(x? |T,t ? ) Where x ? is xj for j=1†¦n, T is a tree with n leaves with sequence j at leaf j, and t ? are tree edge lengths. [17] A few popular stochastic models of evolution include the single parameter Jukes-Cantor (JC) method, Kimura 2-parameter (K2P), Hasegawa-Kishino-Yano (HKY), and Equal-Input.Some s oftware programs, such as Paup*, will automatically use a default model for the tree estimation method chosen. The JC method is the easiest one to comprehend, because it assumes that if a site changes its state, it changes with equal probability to the other states. This is not very realistic, however, as some sites are known to evolve more rapidly than others, and some sites may be invariable and not allowed to change at all. Determining how best to select the appropriate model is a topic of another paper (or papers) as there is no one model that incorporates all mutation rules and patterns across different species and macromolecules. 4, 17, 20] Hidden Markov Models Profile hidden Markov models (HMMs) are a form of Bayesian network that provides statistical models of the consensus structure of a sequence family. Gary Churchill at The Jackson Lab was the first evolutionary geneticist to propose using profile HMMs to model rates of evolution. Many software packages and web services n ow apply HMMs to estimate phylogenetic relationships. [8] In the HMM format, each position in the model corresponds to a site in the sequence alignment. For each position, there are a number of possible states, each of which corresponds to a different rate of evolution.In addition, transitions between all possible rate-states at adjacent positions. Transition probabilities capture any tendency for patterns of rates to occur in successive sites. [2, 4] Assessing Trees Tree estimating algorithms generate one or more optimal trees. This set of possible trees is subjected to a series of statistical tests to evaluate whether one tree is better than another – and if the proposed phylogeny is reasonable. Common methods for assessing trees include the Bootstrap and Jackknife Resampling methods, and analytical methods, such as parsimony, distance, and likelihood.To illustrate how these methods are used, consider the steps involved in a bootstrap analysis. Bootstrap Analysis A bootstra p is a statistical method for assessing trees that takes its name from the fact that it can â€Å"pull itself up by its bootstraps† and generate meaningful statistical distributions from almost nothing. Using bootstrap analysis, distributions that would otherwise be difficult to calculate exactly are estimated by repeated creation and analysis of artificial datasets. In a Non-parametric bootstrap, artificial datasets Molecular Phylogenetics Karen Dowell 11 generated by resampling from original data.In a parametric bootstrap, data is simulated according to hypothesis tested. The objective of any bootstrap analysis is to test whether the whole dataset supports the tree. [1, 4, 17] Figure 14 illustrates the basic steps in any bootstrap analysis. Sample datasets are automatically generated from an original dataset. Trees are then estimated from each sample dataset. The results are compiled and compared to determine a bootstrap consensus tree. Figure 14. Steps in a phylogenetic tr ee bootstrap analysis. [1] Phylogenetic Analysis Tools There are several good online tools and databases that can be used for phylogenetic analysis.These include PANTHER, P-Pod, PFam, TreeFam, and the PhyloFacts structural phylogenomic encyclopedia. Each of these databases uses different algorithms and draws on different sources for sequence information, and therefore the trees estimated by PANTHER, for example, may differ significantly from those generated by P-Pod or PFam. As with all bioinformatics tools of this type, it is important to test different methods, compare the results, then determine which database works best (according to consensus results, not researcher bias) for studies involving different types of datasets.In addition, to the phylogenetic programs already mentioned in this paper, a comprehensive list of more than 350 software packages, web-services, and other resources can be found here: http://evolution. genetics. washington. edu/phylip/software. html. PANTHER ( pantherdb. org) Protein ANalysis Through Evolutionary Relationships, known by its acronym PANTHER, is a library of protein families and subfamilies indexed by function. Panther version 6. 1 contains 5547 protein families. Molecular Phylogenetics Karen Dowell 12It categorizes proteins by evolutionary related proteins (families) and related proteins with same function (subfamilies). [8, 21, 26] PANTHER is composed of both a library and index. The library is a collection of â€Å"books† that represent a protein family as a collection of multiple sequence alignments, HMMs, and a family phylogenetic tree. Functional divergence within the tree is represented by dividing the parent tree into child trees and HMMs based on shared functions. These subfamilies enable database curators to more accurately capture functional divergence of protein sequences as inferred from genomic DNA. 25, 26] PANTHER database entries are annotated to molecular function, biological process and pathway with a proprietary PANTHER/X ontology system, which is supposed to be easier to understand than the more global standard Gene Ontology (GO). Database entries in PANTHER are generated through clustering of UniProt database using a BLAST-based similarity score. Trees are automatically generated based on multiple sequence alignments and parameters of the protein family HMMs using the Tree Inferred from Profile Score (TIPS) clustering algorithm.Scientific curators review all family trees, annotate each tree, and determine how best to divide them into subtrees using a tree-attribute viewer that tabulates annotations for sequences in a tree. In addition, trees and subfamilies are manually cross-checked and validated by curators. [25, 26] P-POD (ortholog. princeton. edu) The Princeton Protein Orthology Database (P-POD) combines results from multiple comparative methods with curated information culled from the literature.Designed to be a resource for experimental biologists seeking evolutionary information on genes on interest, P-POD employs a modular architecture, based on their Generic Model Organism Database (GMOD). P-POD can be accessed from their web service or downloaded to run on local computer systems. [12] P-POD accepts FASTA-formatted protein sequences as input, and performs comparative genomic analyses on those sequences using OrthoMCL and Jaccard clustering methods. The P-POD database contains both phylogenetic information and manually curated experimental results.The site also provides many links to sites rich in human disease and gene information. This tool may be particularly helpful for bioinformaticists and statisticians developing comparative genomic database tools and resources. Pfam (pfam. sanger. ac. uk/) PFam is a collection of protein families represented by multiple sequence alignments and HMMs. It contains models of protein clans, families, domains, and motifs, and uses HMMs representing conserved functional and structural domains. It is a large, widely used, actively curated mature database that has been available online since 1995.Pfam can be used to retrieve the domain architectures for a specific protein by conducting a search using a protein sequence against the Pfam library of HMMs. This database is also helpful for proteomes and protein domain architecture analysis. [6, 8, 24] There are two versions of the Pfam database: Pfam–B is generated automatically from ProDom, using PsiBLAST, an open access bioinformatics tool available through NCBI for identifying weak, but biologically relevant sequence similarities. Pfam-A is hand-curated from custom multiple sequence alignments. Pfam protein domain families are clustered with Mkdom2, and aligned with ProDomAlign.ProDom is a comprehensive set of protein domain families automatically generated from the SWISSPROT and TrEMBL sequence databases. Mkdom2 is a ProDom program used to make ProDom family clusters. Protein domain families in ProDom were aligned using an improved parallelized program called Molecular Phylogenetics Karen Dowell 13 ProDomAlign, developed in C++ using OpenMP. ProDomAlign is based on MultAlign, a program well suited for aligning very large sequence families with thousands of associated sequences. As of early 2008, Pfam matched 72 percent of known proteins sequences, and 95 percent of proteins for which there is a known structure.Within the Pfam database, 75 percent of sequences will have one match to Pfam-A, 19 percent to Pfam-B. There are also two versions of Pfam-A and Pfam-B. Pfam-ls handles global alignments, and Pfam-fs is optimized for local alignments. Interestingly, Pfam entries can be classified as â€Å"unknown,† but that doesn’t mean the protein is undocumented. Unknown entries can be proteins for which some information is known, but it has not been fully researched or cannot be adequately annotated. For example, Pfam entry PFO1816 is a LeucineRich Repeat Variant (LRV), which has a known structure (1LRV ) available in the Protein Databank (pdb. rg). LRV repeat regions, which are found in many different proteins, are often involved in cell adhesion, DNA repair, and hormone reception—but identification of an LRV within a sequence encoding a protein doesn’t specifically reveal the protein’s function. For studies involving a large number of protein searches, it may be more convenient to run Pfam locally on a client machine. The standalone Pfam system requires the HMMER2 software, the Pfam HMM libraries and a couple of additional files from the Pfam website to be installed on the client machine. HMMER is a freely distributable implementation of profile HMM software for protein sequence analysis. ) Once the initial search is complete, researchers can go to the Pfam website to further analyze select number of sequences using additional features on website. [6, 8, 24] TreeFam (TreeFam. org) TreeFam is a curated database of phylogenetic trees and orthology predictions f or all animal gene families that focuses on gene sets from animals with completely sequenced genomes. Orthologs and paralogs are inferred from phylogenetic tree of gene family.Release 4 contains curated trees for 1314 families and automatically generated trees for another 14351 families. [16, 23] Like Pfam, TreeFam is a two-part database: TreeFam-B contains automatically generated trees, and TreeFam-A consists of manually curated trees. To automatically generate trees, an algorithm selects clusters of genes to create TreeFam-B â€Å"seeds† from core species with high-quality reference genome sequences, first using BLAST to rapidly assemble an initial list of possible matches, then HMMER to expand and filter probable sequence matches for each TreeFam B seed family.The filtered alignment is fed into a neighbor-joining algorithm and a tree is constructed based on amino acid mismatch distances. For TreeFam version 4, the most current release, five â€Å"clean† family trees were built for each TreeFam B seed, two using a maximum likelihood tree generated using PHYML (one based on the protein alignment, the other on codon alignment), three using a neighbor joining tree, using different distance measurements based on codon alignments. 16, 23] Scientific curators then manually any correct errors (based on information in the literature) in automatically generated TreeFam-B trees. Curated TreeFam-B trees then become seeds for TreeFam-A trees. Clean TreeFam-A trees are build using three merging algorithms and bootstrapping to find the consensus tree of seven trees: two constrained maximum likelihood trees based on protein and codon alignment, and five unconstrained neighbor-joining trees generated using different distance measurements based on codon alignments.For both TreeFam-B and TreeFam-A families, orthologs and paralogs are inferred only from clean trees using Duplication/Loss Inference (DLI) algorithm that requires a species tree (NCBI taxonomy tree). [16, 23] Molecular Phylogenetics PhyloFacts (phylogenomics. berkeley. edu/phylofacts) Karen Dowell 14 PhyloFacts is an online phylogenomic encyclopedia for protein functional and structural classification. It contains more than 57,000 â€Å"books† for protein superfamilies and structural domains.Each book contains heterogenous data for protein families, including multiple sequence alignments, one or more phylogenetic trees, predicted 3-D protein structures, predicted functional subfamilies, taxonomic distributions, GO annotations, and PFAM domains. HMMs constructed for each family and subfamily permit novel sequences to be classified to different functional classes. [14] Unlike other databases mentioned in this paper, PhyloFacts seeks to correct and clarify annotation errors associated with computational methods for predicting protein function based on sequence homology.It uses a consensus approach that integrates many different prediction methods and sources of experimental data over an evolutionary tree. By applying evolutionary and structural clustering of proteins, PhyloFacts is able to analyze disparate datasets using multiple methods, identify potential errors in database annotations, and provide a mechanism for improving the accuracy of functional annotation in general. [14] PhyloFacts can be used to search for protein structure prediction or functional classification for a particular protein sequence.Researchers may also browse through protein family books and multiple sequence alignments, phylogenetic trees, HMMs and other pertinent information for proteins of interest. This webservice also provides many links to literature and other information sources. [14] Applied Molecular Phylogenetics Molecular phylogenetic studies have many diverse applications. As the amount of publically available molecular sequence data grows and methods for modeling evolution become more sophisticated and accessible, more and more biologists are incorporating phylog enetic analyses into their research trategy. Here’s a sampling of how molecular phylogenetics might be applied. Tracing the evolution of man In one case study, molecular phylogenetic techniques were used to compare and analyze variation in DNA sequences using modern human and Neanderthal mitochondrial DNA (mtDNA). For this study, 206 modern human mtDNAs and parts of two Neanderthal mtDNAs sequences derived from skeletal remains were used to generate an initial dataset. Genetic distance was first estimated using the Jukes-Cantor single parameter model.Then the Kimura 2-Parameter model was used to distinguish between transition (replacement of one purine with another purine or one pyrimidine with another pyrimidine) and transversion (replacement of one purine with a pyrimidine or vice versa) probabilities with Kimura 2parameter model. A phylogenetic tree representing primate evolution was generated using pairwise genetic distances between primate Hypervariable regions I and II of mtDNA. [3] Chasing an epidemic: SARS Using publically available genomic data, it is possible to reconstruct the progression of the SARS epidemic over time and geographically.To conduct this phylogenetic analysis, researchers used the neighborjoining method to construct a phylogenetic tree of spike proteins in various coronaviruses and identify the viral host (a Himalyan palm civet). They then obtained 13 SARs genome sequences with documented information on the date and location of the sample. The neighbor-joining method and a distance matrix based on Jukes-Cantor model, were used to generate an epidemic tree, from which it was possible to identify the origin (date and location) of the virus by observing progression of mutations over time. 3] Molecular Phylogenetics Barking up the right tree Karen Dowell 15 Phylogenetics is increasingly incorporated into biological and biomedical research papers. When the canine genome was published, researchers used sequence data to estimate a co mprehensive phylogeny of the canid family. Figure 15. Phylogenetic Tree of the Canid family This canid family phylogenetic tree is based on 15 kb of exon and intron sequence. It was constructed using the maximum parsimony method and represents the single most parsimonious tree.A good example of how phylogenies are referenced in the literature, this tree includes bootstrap values and Bayesian posterior probability values listed above and below internodes, respectively. Dashes indicate bootstrap values below 50%. In addition, divergence time in millions of years (Myr) is indicated for three nodes. [18] Seeing the Forest from the Trees Molecular phylogenetics is a broad, diverse field with many applications, supported by multiple computational and statistical methods. The sheer volumes of genomic data currently available (and rapidly growing) render molecular phylogenetics a key component of much biological research.Genome-scale studies on gene content, conserved gene order, gene expre ssion, regulatory networks, metabolic pathways, functional genome annotation can all be enriched by evolutionary studies based on phylogenetic statistical analyses. [19, 25 27] Molecular phylogenies have fast become an integral part of biological research, pharmaceutical drug design, and bioinformatics techniques for protein structure prediction and multiple sequence alignment. Although not all molecular biologists and bioinformaticians may be familiar with the techniques describedMolecular Phylogenetics Karen Dowell 16 in this paper, this is a rapidly growing and expanding field and there is ongoing need for novel algorithms to solve complex phylogeny reconstruction problems. References 1. Baldauf, SL (2003) â€Å"Phylogeny for the faint of heart: a tutorial. † Trends in Genetics, 19(6):345-351. 2. Brown, D, K Sjolander (2006) â€Å"Functional Classification Using Phylogenomic Inference. † PLos Computational Biology, 2(6):0479-0483. 3. Cristianini, N, and M Hahn (2007 ) Introduction to Computational Genomics: A Case Studies Approach.Cambridge University Press: Cambridge. 4. 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A University of Washington site formerly supported by the National Science Foundation. http://www. evolution. genetics. washington. edu/phylip/software. tml TreeFam Tree Families Database. http://wwww. treefam. org Protein Analysis Through Evolutionary Relationships (PANTHER) Classification System. http://www. pantherdb. org. 29. Pfam Database of Protein Families. http://pfam. sanger. ac. uk 30. Princeton Protein Orthology Database (P-POD). http://ppod. princeton. edu 31. Wikipedia. http://en. wikipedia. org/wiki/Tree_of_life(science) Cover Page The cover image is from a phylogeny of canid species that appeared in Lindblad-Toh et al, 2005. [18]